Hou Yan, Wang Yaoqin, Xu Suming, Qi Gaimei, Wu Xueqing

The Second Hospital of Shanxi Medical University Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.| Center of Reproductive Medicine, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030013, P.R. China.| Center of Reproductive Medicine, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030013, P.R. China.| Center of Reproductive Medicine, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030013, P.R. China.| The Second Hospital of Shanxi Medical University Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Mol Med Rep. 2019 Jul;20(1):281-291. doi: 10.3892/mmr.2019.10253. Epub 2019 May

Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women of reproductive age. MicroRNAs (miRNAs or miRs) serve important roles in the physiological and pathological process of PCOS. To identify PCOSassociated miRNAs, the dataset GSE84376 was extracted from the Gene Expression Omnibus database. Differentially expressed miRNAs (DEmiRNAs) were obtained from GeneCloud Biotechnology Information and potential target genes were predicted using TargetScan, DIANAmicroTCDS, miRDB and miRTarBase tools. Gene Ontology enrichment analysis was performed using Metascape and a proteinprotein interaction network was constructed using Cytoscape. Transcription factors were obtained from FunRich. DEmiRNAs were verified by reverse transcriptionquantitative PCR. At the screening phase, there were seven DEmiRNAs in the PCOS group not present in the control group. In total, 935 target genes were identified, which are involved in the development and maturation of oocytes. Mitogenactivated protein kinase 1, phosphatase and tensin homolog, cAMP responsive element binding protein 1, signal transducer and activator of transcription 3, interferon gamma, Fmsrelated tyrosine kinase 1, transcription factor p65, insulin receptor substrate 1, DnaJ homolog superfamily C member 10 and casein kinase 2 alpha 1 were identified as the top 10 hub genes in the proteinprotein interaction network. Specificity protein 1 was the most enriched transcription factor. At the validation phase, the levels of Homo sapiens (hsa)miR3188 and hsamiR3135b were significantly higher in the PCOS group than in the control group. In addition, the expression level of hsamiR3135b was significantly correlated with the number of oocytes retrieved, the fertilization rate and the cleavage rate (P<0.05). The present bioinformatics study on miRNAs may offer a novel understanding of the mechanism of PCOS, and may serve to identify novel miRNA therapeutic targets.