NDST1: Polycystic Ovarian Syndrome Database

Gene Information

Gene Symbol

NDST1

Aliases

HSST, MRT46, NST1

Entrez Gene ID

3340

Gene Name

N-deacetylase and N-sulfotransferase 1

Chromosomal Location

5q33.1

HGNC ID

HGNC:7680

Summary

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

RefSeq DNA

NG_041806.1

RefSeq mRNA

NM_001543.5, NM_001301063.2

e!Ensembl

Gene	ENSG00000070614
Transcript	ENST00000522491, ENST00000519157, ENST00000523767, ENST00000518299, ENST00000261797
Protein	ENSP00000429966, ENSP00000427813, ENSP00000428604, ENSP00000430258, ENSP00000261797

Gene Ontology (GO)

Show/Hide all(12 )

GO ID	Ontology	Function	Evidence	Reference
GO:0015012	Biological process	Heparan sulfate proteoglycan biosynthetic process	IBA	21873635
GO:0015012	Biological process	Heparan sulfate proteoglycan biosynthetic process	IDA	9915799
GO:0015014	Biological process	Heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IBA	21873635
GO:0015014	Biological process	Heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IDA	9915799
GO:0005794	Cellular component	Golgi apparatus	IBA	21873635
GO:0005515	Molecular function	Protein binding	IPI	18337501
GO:0015016	Molecular function	[heparan sulfate]-glucosamine N-sulfotransferase activity	IBA	21873635
GO:0015016	Molecular function	[heparan sulfate]-glucosamine N-sulfotransferase activity	IDA	9915799
GO:0019213	Molecular function	Deacetylase activity	IBA	21873635
GO:0034483	Molecular function	Heparan sulfate sulfotransferase activity	IBA	21873635
GO:0042328	Molecular function	Heparan sulfate N-acetylglucosaminyltransferase activity	IBA	21873635
GO:0042328	Molecular function	Heparan sulfate N-acetylglucosaminyltransferase activity	IDA	9915799

Protein Information

Protein Name

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, HSNST 1, N-Deacetylase-N-sulfotransferase 1, N-HSST 1, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1, N-deacetylase/N-sulfotransferase 1, N-heparan sulfate sulfotransferase 1, NDST-1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, glucosaminyl N-deacetylase/N-sulfotransferase 1, heparan sulfate-N-deacetylase/N-sulfotransferase, heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase 1

Function

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan

Refseq Proteins

NP_001534.1, NP_001287992.1

UniProt

P52848

PDB

1NST

Pfam

Pfam Accession	Pfam ID
PF12062	HSNSD
PF00685	Sulfotransfer_1